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  1. We report a minimal microtubule-based motile system displaying signatures of unconventional diffusion. The system consists of a single model cargo driven by an ensemble of N340K NCD motors along a single microtubule. Despite the absence of cytosolic or cytoskeleton complexity, the system shows complex behavior, characterized by sub-diffusive motion for short time lag scales and linear mean squared displacement dependence for longer time lags. The latter is also shown to have non-Gaussian character and cannot be ascribed to a canonical diffusion process. We use single particle tracking and analysis at varying temperatures and motor concentrations to identify the origin of these behaviors as enzymatic activity of mutant NCD. Our results show that signatures of non-Gaussian diffusivities can arise as a result of an active process and suggest that some immotility of cargos observed in cells may reflect the ensemble workings of mechanochemical enzymes and need not always reflect the properties of the cytoskeletal network or the cytosol. 
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